Mode of binding of MAP2 onto microtubule lattice

The phosphatidylinositol-binding site of microtubule-associated protein MAP2.

Recent evidence [Surridge and Burns, Biochemistry (1994) 33, 8051-8057] on the interaction of native and recombinant tau, recombinant MAP2c, and native MAP2 with vesicles prepared from phosphatidylinositol (PtdIns) and other phospholipids demonstrate that MAP2 differs from MAP2c and from tau in having a high-affinity PtdIns-binding site. The location of this site within the MAP2-specific insert...

Sorting mechanisms of Tau and MAP2 in neurons: Suppressed axonal transit of MAP2 and locally regulated microtubule binding

Tau is abundant in the axon, whereas MAP2 is found in the cell body and dendrites. To understand their differential localization, we performed transfection studies on primary cultured neurons using tagged tau, MAP2, MAP2C, and their chimeric/deletion mutants. We found that MAP2 was prevented from entering the axon by its N-terminal projection domain and that microtubule binding of tau was stron...

An isoform of microtubule-associated protein 2 (MAP2) containing four repeats of the tubulin-binding motif.

Microtubule-associated protein 2 (MAP2) exists in both high- and low-molecular mass isoforms, each of which has a tubulin-binding domain consisting of 3 imperfect tandem repeats of 31 amino acids containing a more highly conserved 18 amino acid 'core' sequence. We describe here a novel form of low molecular mass MAP2 (MAP2c) that contains an additional 4th repeat of this tubulin-binding motif. ...

Specific Binding of Dhea to the N-terminal of the Microtubule-associated Protein Map2

Phosphorylation determines the binding of microtubule-associated protein 2 (MAP2) to microtubules in living cells

The influence of phosphorylation on the binding of microtubule-associated protein 2 (MAP2) to cellular microtubules was studied by microinjecting MAP2 in various phosphorylation states into rat-1 fibroblasts, which lack endogenous MAP2. Conventionally prepared brain MAP2, containing 10 mol of endogenous phosphate per mol (MAP2-P10), was completely bound to cellular microtubules within 2-3 min a...